![]() Where the wall shear stress is low or oscillatory ( 1). Occurs at sites where the blood flow is slow or disturbed, and It is well-known that atherosclerosis preferentially In conclusion, the results of the present study indicate that LSS increases the level of autophagy, which may be through a Bcl‑2/Beclin‑1‑dependent mechanism, which serves a protective role against LSS‑induced apoptosis. Pretreatment of HUVECs with CQ markedly increased LSS‑induced apoptosis, which was associated with an increased expression of Bax and a decreased expression of Bcl‑2. In addition, it was observed that HUVECs treated with LSS underwent apoptotic death, by monitoring the rate of apoptosis and the expression of apoptosis regulator BAX (Bax) and apoptosis regulator Bcl‑2 (Bcl‑2) (by flow cytometry and WB) and the LSS‑induced apoptosis in HUVECs, that was significantly alleviated by pretreatment with rapamycin, partially via a decrease in the level of Bax and an increase in the level of Bcl‑2. Furthermore, the level of p62 decreased when autophagy was induced by rapamycin, and increased when autophagy was inhibited by chloroquine (CQ), which indicated that LSS may serve an important role in inducing autophagy flux. The results revealed that LSS increased the formation of autophagosomes and MAP1 light chain 3‑like protein (LC3) puncta (as demonstrated by transmission electron microscopy and immunofluorescence), and the protein levels of Beclin‑1 and LC3II decreased the expression of p62. Therefore, in the present study, the level and role of autophagy in human umbilical vein endothelial cells (HUVECs) exposed to LSS was examined. The effect of autophagy and its association with apoptosis, in the development of atherosclerosis, remains controversial. However, whether autophagy participates in LSS‑induced atherosclerosis remains unclear. Autophagy has been reported to be involved in the development of atherosclerosis. Low shear stress (LSS) is a well‑established risk factor resulting in endothelial apoptosis and atherosclerosis. ![]()
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